A colorimetric and ratiometric fluorescent probe for thiols and its bioimaging applications.

نویسندگان

  • Baocun Zhu
  • Xiaoling Zhang
  • Yamin Li
  • Pengfei Wang
  • Hongyan Zhang
  • Xiaoqing Zhuang
چکیده

A naphthalimide-based colorimetric fluorescent probe containing a disulfide group was designed and synthesized, which could detect the physiological level of GSH quantitatively by a ratiometric fluorescence method and was successfully applied to the imaging of thiols in living HeLa cells.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A thiol-specific fluorescent probe and its application for bioimaging.

A fluorescent probe based on fluorescein displays an excellent selectivity and sensitivity for thiols and its application for bioimaging is described.

متن کامل

A Novel Dicyanoisophorone-Based Ratiometric Fluorescent Probe for Selective Detection of Cysteine and Its Bioimaging Application in Living Cells.

A selective and ratiometric turn-on fluorescent probe was designed and synthesized by using a novel dicyanoisophorone-based derivative and acrylate moiety. The probe displayed high stability and good selectivity to cysteine (Cys) over homocysteine (Hcy) and glutathione (GSH). It also exhibited rapid response to Cys within 180 s. Most importantly, the fluorescence intensity ratio at 590 and 525 ...

متن کامل

A low dose, highly selective and sensitive colorimetric and fluorescent probe for biothiols and its application in bioimaging.

A new colorimetric and fluorescent probe was reported, which can be used in a very low dosage (<20 nM) for rapid, highly selective and sensitive detection of biothiols.

متن کامل

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide.

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R(2) = 0.9867) versus hydrogen sulfide concentration in the range of 1-12 μM was obtained. This probe exhibited a remarkable fluorescence respo...

متن کامل

Real-time detection of hypochlorite in tap water and biological samples by a colorimetric, ratiometric and near-infrared fluorescent turn-on probe.

In this paper, we report a highly selective and sensitive ratiometric NIR fluorescent probe that can be used for real-time detection of the biologically important hypochlorite with colorimetric and significant NIR fluorescent turn-on signal changes at NIR excitation wavelength. In addition, experiments showed that this probe can be applied to detect hypochlorite in tap water, serum samples, and...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 46 31  شماره 

صفحات  -

تاریخ انتشار 2010